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mouse monoclonal anti osteocalcin antibodies  (R&D Systems)


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    Structured Review

    R&D Systems mouse monoclonal anti osteocalcin antibodies
    Mouse Monoclonal Anti Osteocalcin Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+osteocalcin+monoclonal+antibody/Human+Osteocalcin+Antibody/pmc13068806-148-34-43
    Average 93 stars, based on 30 article reviews
    mouse monoclonal anti osteocalcin antibodies - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis.
    Article Snippet: At confluency, cells were fixed for 20 min in 4% paraformaldehyde (Sigma Aldrich, Saint-Quentin Fallavier, France) with 0.15% picric acid added to it as requested by type-I collagen antibody manufacturer, then washed with DPBS. .. Cells were permeabilized with 3% BSA (Sigma Aldrich, Saint-Quentin Fallavier, France) plus 0.1% Triton X100 (Acros Organics, Thermo Fisher Scientific, Illkirch, France) for 45 min then incubated overnight at 4°C with rabbit anti-human type-I collagen monoclonal antibody (clone H-197, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-human osteocalcin monoclonal antibody (clone 190125, R&D systems, Lille, France). .. Cells were rinsed in DPBS and blocked with 3 % BSA for 30 min. After washing, cells were incubated with goat anti-rabbit biotinylated antibody (diluted 1/50 in 1% BSA) (Vector, Burlingame, California) for 30 min, rinsed in DPBS and incubated with a solution of streptavidine-Alexafluor®488 (diluted at 1/200, Molecular Probes, Thermo Fisher Scientific, Illkirch, France).

    Article Title: Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology
    Article Snippet: .. After 20 days of induction, cells were fixed in 4% FA and incubated with the Mouse Anti-human osteocalcin monoclonal antibody (1:500; R&D, cat# MAB1419) followed by incubation with anti-mouse DyLightTM 594 secondary antibody (1:500) and 1 μM Hoechst 33,342 (Life Technologies). ..

    Article Title: A biomaterials approach to influence stem cell fate in injectable cell-based therapies
    Article Snippet: .. Cells were permeabilised using 0.1% (w/v) Triton-X 100 in PBS (Sigma-Aldrich) for 30 min. Non-specific binding sites were blocked by incubation in 10% ( v /v) normal donkey serum (D9663; Sigma-Aldrich) and 1% bovine serum albumin (BSA) in PBS for 1 h. Cells were then incubated with Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB1419; R&D Systems) diluted in 1% (w / v) BSA in PBS at 10 μg/ml for 3 h at room temperature. ..

    Article Title: Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology.
    Article Snippet: .. After 20 days of induction, cells were fixed in 4% FA and incubated with the Mouse Anti-human osteocalcin monoclonal antibody (1:500; R&D, cat# MAB1419) followed by incubation with anti-mouse DyLightTM 594 secondary antibody (1:500) and 1 μM Hoechst 33,342 (Life Technologies). ..

    Article Title: In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis
    Article Snippet: At confluency, cells were fixed for 20 min in 4% paraformaldehyde (Sigma Aldrich, Saint-Quentin Fallavier, France) with 0.15% picric acid added to it as requested by type-I collagen antibody manufacturer, then washed with DPBS. .. Cells were permeabilized with 3% BSA (Sigma Aldrich, Saint-Quentin Fallavier, France) plus 0.1% Triton X100 (Acros Organics, Thermo Fisher Scientific, Illkirch, France) for 45 min then incubated overnight at 4 °C with rabbit anti-human type-I collagen monoclonal antibody (clone H-197, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-human osteocalcin monoclonal antibody (clone 190125, R&D systems, Lille, France). .. Cells were rinsed in DPBS and blocked with 3% BSA for 30 min. After washing, cells were incubated with goat anti-rabbit biotinylated antibody (diluted 1/50 in 1% BSA) (Vector, Burlingame, California) for 30 min, rinsed in DPBS and incubated with a solution of streptavidine-Alexafluor ® 488 (diluted at 1/200, Molecular Probes, Thermo Fisher Scientific, Illkirch, France).

    Binding Assay:

    Article Title: A biomaterials approach to influence stem cell fate in injectable cell-based therapies
    Article Snippet: .. Cells were permeabilised using 0.1% (w/v) Triton-X 100 in PBS (Sigma-Aldrich) for 30 min. Non-specific binding sites were blocked by incubation in 10% ( v /v) normal donkey serum (D9663; Sigma-Aldrich) and 1% bovine serum albumin (BSA) in PBS for 1 h. Cells were then incubated with Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB1419; R&D Systems) diluted in 1% (w / v) BSA in PBS at 10 μg/ml for 3 h at room temperature. ..



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